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aea hydrolysis  (Tocris)


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    Structured Review

    Tocris aea hydrolysis
    Aea Hydrolysis, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aea+hydrolysis/Anandamide/pmc12218337-172-0-43
    Average 93 stars, based on 355 article reviews
    aea hydrolysis - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: Endocannabinoids may mediate the ability of (n-3) fatty acids to reduce ectopic fat and inflammatory mediators in obese Zucker rats.
    Article Snippet: .. AEA hydrolysis (by FAAH) was measured by incubating the 10,000 3 g membrane fraction of tissues (70 mg per sample) in Tris-HCl 50 mmol/L, at pH 9.0–10.00 at 378C for 30 min, with synthetic N-arachidonoyl-[14C]-ethanolamine (110 mCi/mmol, ARC) properly diluted with AEA (Tocris Bioscience). ..

    Article Title: Early Low-Fat Diet Enriched With Linolenic Acid Reduces Liver Endocannabinoid Tone and Improves Late Glycemic Control After a High-Fat Diet Challenge in Mice.
    Article Snippet: .. AEA hydrolysis was measured by incubating the 10,000 x g liver membrane fraction (70-100 μg/sample) in Tris-HCl l50 mM, at pH 9-10 at 37°C for 30 min, with synthetic N-arachidonoyl-[14C]-ethanolamine (55 mCi/mmol, ARC St. Louis, MO, USA) properly diluted with AEA (Tocris Bioscience, Avonmouth, Bristol, UK) to the final concentration of 2 μM. ..

    Article Title: Deciphering the interaction between N -palmitoyl-D-glucosamine and the endocannabinoidome
    Article Snippet: .. AEA hydrolysis was measured by incubating the 10,000 g membrane fraction of whole rat brain (70 μg/sample) in Tris-HCl 50 mM, at pH 9.0–10.0 at 37 °C for 30 min, with N -arachidonoyl-[ 14 C]-ethanolamine (110 mCi/mmol, ARC) properly diluted with unlabeled AEA (Tocris Bioscience), containing vehicle or increasing concentrations of PGA. ..

    Concentration Assay:

    Article Title: Early Low-Fat Diet Enriched With Linolenic Acid Reduces Liver Endocannabinoid Tone and Improves Late Glycemic Control After a High-Fat Diet Challenge in Mice.
    Article Snippet: .. AEA hydrolysis was measured by incubating the 10,000 x g liver membrane fraction (70-100 μg/sample) in Tris-HCl l50 mM, at pH 9-10 at 37°C for 30 min, with synthetic N-arachidonoyl-[14C]-ethanolamine (55 mCi/mmol, ARC St. Louis, MO, USA) properly diluted with AEA (Tocris Bioscience, Avonmouth, Bristol, UK) to the final concentration of 2 μM. ..



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    aea hydrolysis  (Tocris)
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    Tocris aea hydrolysis
    Aea Hydrolysis, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    amc aea hydrolysis  (Molecular Devices LLC)
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    CB1 and PPARα mediate the antinociceptive effects in FABP5 cKO mice. ( A ) Levels of <t>AEA,</t> PEA, OEA, and 2-AG in DRGs of male and female FABP5 Flox and FABP5 cKO mice at baseline and 4 h after intraplantar carrageenan injection. ** p < 0.01; *** p < 0.001 vs WT and FABP5 Flox calculated using two-way ANOVA followed by Bonferroni post-hoc test (n = 5). ( B ) Percent expression of CB1, PPARα, FAAH, NAPE-PLD, and ABHD4 in DRGs of male and female FABP5 cKO mice relative to FABP5 Flox controls. ( C ) FAAH and NAPE-PLD activity in DRGs. Left: DRGs were incubated with the selective FAAH substrate <t>AMC-AEA</t> (100 µM). Right: DRGs were incubated with the phospholipase A1 substrate PED-A1 (10 µM) in the presence or absence of the selective NAPE-PLD inhibitor LEI-401 (30 µM) (n = 3). ( D ) Thermal withdrawal latencies in FABP5 cKO mice receiving vehicle, AM251 (3 mg/kg, i.p.), or GW6471 (4 mg/kg, i.p.). *** p < 0.001 calculated using one-way ANOVA followed by Dunnett’s post-hoc test (n = 6).
    Amc Aea Hydrolysis, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    aea hydrolysis inhibitor urb597  (Cayman Chemical)
    94
    Cayman Chemical aea hydrolysis inhibitor urb597
    FIGURE 2 Increased <t>anandamide</t> (AEA) signalling did not significantly alter auditory fear memory expression and extinction in males. (a–f) Treatment with the AEA hydrolysis inhibitor <t>URB597,</t> the CB1 antagonist AM251 or their combination (URB597 + AM251) did not significantly alter freezing or darting behaviour in male rats, although URB597 showed a trend towards reducing conditioned freezing as compared to the vehicle group during the late phases of extinction training and retrieval and significantly reduced freezing compared to URB597 + AM251 (at extinction retrieval). Percentage of freezing during auditory fear conditioning (a), extinction training (b) and extinction retrieval (c). Number of darting eventsmin−1 (dartmin−1) during auditory fear conditioning (d), extinction training (e), and extinction retrieval (f). Vehicle, n = 18; URB597, n = 17; AM251, n = 12; URB597 + AM251, n = 14. Data are expressed as mean ± SEM. *P < 0.05 main effect of drug; ◆P < 0.05 versus URB597 + AM251
    Aea Hydrolysis Inhibitor Urb597, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aea+hydrolysis/URB597/pm33314038-102-1-8
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    Image Search Results


    CB1 and PPARα mediate the antinociceptive effects in FABP5 cKO mice. ( A ) Levels of AEA, PEA, OEA, and 2-AG in DRGs of male and female FABP5 Flox and FABP5 cKO mice at baseline and 4 h after intraplantar carrageenan injection. ** p < 0.01; *** p < 0.001 vs WT and FABP5 Flox calculated using two-way ANOVA followed by Bonferroni post-hoc test (n = 5). ( B ) Percent expression of CB1, PPARα, FAAH, NAPE-PLD, and ABHD4 in DRGs of male and female FABP5 cKO mice relative to FABP5 Flox controls. ( C ) FAAH and NAPE-PLD activity in DRGs. Left: DRGs were incubated with the selective FAAH substrate AMC-AEA (100 µM). Right: DRGs were incubated with the phospholipase A1 substrate PED-A1 (10 µM) in the presence or absence of the selective NAPE-PLD inhibitor LEI-401 (30 µM) (n = 3). ( D ) Thermal withdrawal latencies in FABP5 cKO mice receiving vehicle, AM251 (3 mg/kg, i.p.), or GW6471 (4 mg/kg, i.p.). *** p < 0.001 calculated using one-way ANOVA followed by Dunnett’s post-hoc test (n = 6).

    Journal: Scientific Reports

    Article Title: FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory pain

    doi: 10.1038/s41598-022-13284-0

    Figure Lengend Snippet: CB1 and PPARα mediate the antinociceptive effects in FABP5 cKO mice. ( A ) Levels of AEA, PEA, OEA, and 2-AG in DRGs of male and female FABP5 Flox and FABP5 cKO mice at baseline and 4 h after intraplantar carrageenan injection. ** p < 0.01; *** p < 0.001 vs WT and FABP5 Flox calculated using two-way ANOVA followed by Bonferroni post-hoc test (n = 5). ( B ) Percent expression of CB1, PPARα, FAAH, NAPE-PLD, and ABHD4 in DRGs of male and female FABP5 cKO mice relative to FABP5 Flox controls. ( C ) FAAH and NAPE-PLD activity in DRGs. Left: DRGs were incubated with the selective FAAH substrate AMC-AEA (100 µM). Right: DRGs were incubated with the phospholipase A1 substrate PED-A1 (10 µM) in the presence or absence of the selective NAPE-PLD inhibitor LEI-401 (30 µM) (n = 3). ( D ) Thermal withdrawal latencies in FABP5 cKO mice receiving vehicle, AM251 (3 mg/kg, i.p.), or GW6471 (4 mg/kg, i.p.). *** p < 0.001 calculated using one-way ANOVA followed by Dunnett’s post-hoc test (n = 6).

    Article Snippet: AMC-AEA hydrolysis was quantified on a SpectraMax i3x plate reader (Molecular Dynamics) using excitation and emission wavelengths of 360 and 465 nm, respectively.

    Techniques: Injection, Expressing, Activity Assay, Incubation

    FIGURE 2 Increased anandamide (AEA) signalling did not significantly alter auditory fear memory expression and extinction in males. (a–f) Treatment with the AEA hydrolysis inhibitor URB597, the CB1 antagonist AM251 or their combination (URB597 + AM251) did not significantly alter freezing or darting behaviour in male rats, although URB597 showed a trend towards reducing conditioned freezing as compared to the vehicle group during the late phases of extinction training and retrieval and significantly reduced freezing compared to URB597 + AM251 (at extinction retrieval). Percentage of freezing during auditory fear conditioning (a), extinction training (b) and extinction retrieval (c). Number of darting eventsmin−1 (dartmin−1) during auditory fear conditioning (d), extinction training (e), and extinction retrieval (f). Vehicle, n = 18; URB597, n = 17; AM251, n = 12; URB597 + AM251, n = 14. Data are expressed as mean ± SEM. *P < 0.05 main effect of drug; ◆P < 0.05 versus URB597 + AM251

    Journal: British journal of pharmacology

    Article Title: Sex-dependent effects of endocannabinoid modulation of conditioned fear extinction in rats.

    doi: 10.1111/bph.15341

    Figure Lengend Snippet: FIGURE 2 Increased anandamide (AEA) signalling did not significantly alter auditory fear memory expression and extinction in males. (a–f) Treatment with the AEA hydrolysis inhibitor URB597, the CB1 antagonist AM251 or their combination (URB597 + AM251) did not significantly alter freezing or darting behaviour in male rats, although URB597 showed a trend towards reducing conditioned freezing as compared to the vehicle group during the late phases of extinction training and retrieval and significantly reduced freezing compared to URB597 + AM251 (at extinction retrieval). Percentage of freezing during auditory fear conditioning (a), extinction training (b) and extinction retrieval (c). Number of darting eventsmin−1 (dartmin−1) during auditory fear conditioning (d), extinction training (e), and extinction retrieval (f). Vehicle, n = 18; URB597, n = 17; AM251, n = 12; URB597 + AM251, n = 14. Data are expressed as mean ± SEM. *P < 0.05 main effect of drug; ◆P < 0.05 versus URB597 + AM251

    Article Snippet: The AEA hydrolysis inhibitor URB597 (0.3 mg kg−1; Cayman Chemical, Cedarlane®, Burlington, ON, Canada), the 2-AG hydrolysis inhibitor MJN110 (10 mg kg−1; provided by B.F. Cravatt), the CB1 antagonist/inverse agonist AM251 (1 mg kg−1; Tocris, Cedarlane®, F IGURE 1 Sexually divergent expression of fear responses during the auditory fear conditioning paradigm. (a) Schematic representation of the experimental design. (b–d) Except during conditioned stimulus (CS) presentations at extinction training (c), male rats consistently showed higher freezing behaviour than females during the auditory fear conditioning paradigm.

    Techniques: Expressing

    FIGURE 4 Increased anandamide (AEA) signalling at TRPV1s augmented freezing behaviour at extinction training and retrieval in females. (a–f) Treatment with the AEA hydrolysis inhibitor URB597 with concurrent blockade of CB1 receptors with the antagonist AM251 (URB597 + AM251) induced fear generalization, impaired within-session extinction and extinction retrieval. These effects were mediated by AEA signalling at TRPV1s, as they were completely blocked by concomitant injection with theTRPV1 antagonist capsazepine (CPZ) (URB597 + AM251 + CPZ). Furthermore, treatment with URB597 alone induced an overall reduction of darting behaviour during conditioned stimulus (CS) presentation at extinction training compared to the vehicle group. Percentage of freezing during auditory fear conditioning (a), extinction training (b) and extinction retrieval (c). Number of darting eventsmin−1 (dartmin−1) during auditory fear conditioning (d), extinction training (e) and extinction retrieval (f). Vehicle, n = 20; URB597, n = 18; AM251, n = 14; URB597 + AM251, n = 13; URB597 + AM251 + CPZ, n = 10. Data are expressed as mean ± SEM. *P < 0.05 versus vehicle; •P < 0.05 versus URB597; #P < 0.05 versus AM251; ◆P < 0.05 versus URB597 + AM251 + CPZ. Horizontal line below the star indicates a main effect of drug: P < 0.05, URB597 + AM251 group versus vehicle group (c) and URB597 group versus vehicle group (e)

    Journal: British journal of pharmacology

    Article Title: Sex-dependent effects of endocannabinoid modulation of conditioned fear extinction in rats.

    doi: 10.1111/bph.15341

    Figure Lengend Snippet: FIGURE 4 Increased anandamide (AEA) signalling at TRPV1s augmented freezing behaviour at extinction training and retrieval in females. (a–f) Treatment with the AEA hydrolysis inhibitor URB597 with concurrent blockade of CB1 receptors with the antagonist AM251 (URB597 + AM251) induced fear generalization, impaired within-session extinction and extinction retrieval. These effects were mediated by AEA signalling at TRPV1s, as they were completely blocked by concomitant injection with theTRPV1 antagonist capsazepine (CPZ) (URB597 + AM251 + CPZ). Furthermore, treatment with URB597 alone induced an overall reduction of darting behaviour during conditioned stimulus (CS) presentation at extinction training compared to the vehicle group. Percentage of freezing during auditory fear conditioning (a), extinction training (b) and extinction retrieval (c). Number of darting eventsmin−1 (dartmin−1) during auditory fear conditioning (d), extinction training (e) and extinction retrieval (f). Vehicle, n = 20; URB597, n = 18; AM251, n = 14; URB597 + AM251, n = 13; URB597 + AM251 + CPZ, n = 10. Data are expressed as mean ± SEM. *P < 0.05 versus vehicle; •P < 0.05 versus URB597; #P < 0.05 versus AM251; ◆P < 0.05 versus URB597 + AM251 + CPZ. Horizontal line below the star indicates a main effect of drug: P < 0.05, URB597 + AM251 group versus vehicle group (c) and URB597 group versus vehicle group (e)

    Article Snippet: The AEA hydrolysis inhibitor URB597 (0.3 mg kg−1; Cayman Chemical, Cedarlane®, Burlington, ON, Canada), the 2-AG hydrolysis inhibitor MJN110 (10 mg kg−1; provided by B.F. Cravatt), the CB1 antagonist/inverse agonist AM251 (1 mg kg−1; Tocris, Cedarlane®, F IGURE 1 Sexually divergent expression of fear responses during the auditory fear conditioning paradigm. (a) Schematic representation of the experimental design. (b–d) Except during conditioned stimulus (CS) presentations at extinction training (c), male rats consistently showed higher freezing behaviour than females during the auditory fear conditioning paradigm.

    Techniques: Injection

    FIGURE 6 Sex-dependent effects of auditory fear extinction training on endocannabinoid brain levels. anandamide (AEA) and 2-arachidonoyl glycerol (2-AG) brain levels (pmolg−1 tissue and nmolg−1 tissue, respectively) in the amygdala (AMY; a, b; for AEA and 2-AG: males No-Ext, n = 10, males Ext, n = 11, females No-Ext, n = 10, females Ext, n = 10), prefrontal cortex (PFC; c, d; for AEA and 2-AG: males No-Ext, n = 10, males Ext, n = 11, females No-Ext, n = 10, females Ext, n = 10), dorsal periaqueductal grey (dPAG; e, f; for AEA and 2-AG: males No-Ext, n = 8, males Ext, n = 9, females No-Ext, n = 9, females Ext, n = 9) and ventral periaqueductal grey (vPAG; g, h; for AEA and 2-AG: males No-Ext, n = 8, males Ext, n = 11, females No-Ext, n = 8, females Ext, n = 10) in male and female rats immediately after the extinction training session (Ext group) or in control groups only exposed to the extinction context without the conditioned stimulus (CS) presentations (No-Ext group). Data are expressed as mean ± SEM. *P < 0.05

    Journal: British journal of pharmacology

    Article Title: Sex-dependent effects of endocannabinoid modulation of conditioned fear extinction in rats.

    doi: 10.1111/bph.15341

    Figure Lengend Snippet: FIGURE 6 Sex-dependent effects of auditory fear extinction training on endocannabinoid brain levels. anandamide (AEA) and 2-arachidonoyl glycerol (2-AG) brain levels (pmolg−1 tissue and nmolg−1 tissue, respectively) in the amygdala (AMY; a, b; for AEA and 2-AG: males No-Ext, n = 10, males Ext, n = 11, females No-Ext, n = 10, females Ext, n = 10), prefrontal cortex (PFC; c, d; for AEA and 2-AG: males No-Ext, n = 10, males Ext, n = 11, females No-Ext, n = 10, females Ext, n = 10), dorsal periaqueductal grey (dPAG; e, f; for AEA and 2-AG: males No-Ext, n = 8, males Ext, n = 9, females No-Ext, n = 9, females Ext, n = 9) and ventral periaqueductal grey (vPAG; g, h; for AEA and 2-AG: males No-Ext, n = 8, males Ext, n = 11, females No-Ext, n = 8, females Ext, n = 10) in male and female rats immediately after the extinction training session (Ext group) or in control groups only exposed to the extinction context without the conditioned stimulus (CS) presentations (No-Ext group). Data are expressed as mean ± SEM. *P < 0.05

    Article Snippet: The AEA hydrolysis inhibitor URB597 (0.3 mg kg−1; Cayman Chemical, Cedarlane®, Burlington, ON, Canada), the 2-AG hydrolysis inhibitor MJN110 (10 mg kg−1; provided by B.F. Cravatt), the CB1 antagonist/inverse agonist AM251 (1 mg kg−1; Tocris, Cedarlane®, F IGURE 1 Sexually divergent expression of fear responses during the auditory fear conditioning paradigm. (a) Schematic representation of the experimental design. (b–d) Except during conditioned stimulus (CS) presentations at extinction training (c), male rats consistently showed higher freezing behaviour than females during the auditory fear conditioning paradigm.

    Techniques: Control